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Chilton FH
Sections were washed in PBS three times for 5 min each and were incubated with secondary antibody for 45 min
Excess M9 was aspirated and the remaining pellet was resuspended in 20 L of molecular-grade water and 20 L of 2 SDS sample buffer
Per standard laboratory protocols and manufacturer recommendations (such as those from Hospira), bacteriostatic water should be discarded 28 days after the first puncture
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