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Purified MDH (~1 mL) was dialyzed for 4 h in 1 L buffer 2 (20 mM MOPS-KOH pH 7.5, 150 mM NaCl) containing 10 mM EDTA followed by three times dialysis against 1 L of buffer 2

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Clamshells Lie on your side with hips and knees bent at about 45 degrees, feet stacked

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Consistent sleep and wake times matter more than total hours in bed

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